VisANT-Predictome Interaction Statistics

In addition to the computational predicted interactions produced in our Lab, Predictome database also collects experimentally determined ones by either directly importing from literatures, or by synchronizing with existing interaction databases such as Biogrid, MINT,  BIND, MIPS, IntAct, HPRD etc. The integration of the interaction is gene-based, meaning that two protein nodes will be recognized as a single protein, if the same gene encodes them. This decision not to distinguish gene nodes from protein nodes was based primarily on the following observations:

  • Independently created interaction databases often use different naming systems for proteins/genes. For example, MINT uses a proteins UniProt id in protein-protein interaction for Homo sapiens which, however, is represented using genes HGNC (HUGO Gene Nomenclature Committee) id in Biogrid.
  • Genetic associations very often need to be compared to protein-protein interactions.
  • High throughput gene expression results must often be mapped onto a protein-protein interaction network or pathway.

Because genes often encode multiple proteins, using gene id to represent both gene and protein is a natural choice; otherwise, uncertainty may arise when integrating interaction data. For example, for a gene with two splice variants, the variant to use when integrating interactions between the MINT (protein-based) and Biogrid databases (gene-based) will be ambiguous. The shortcoming of this solution is that certain splice variants may only share a subset of interactions. This dilemma can be overcome if alternative splicing knowledge is represented using a metanode, an undergoing process of this project.
 

Summary
Total*:1151438 Computational predicted:300292 Experimental determined:851146
Species Supported:111
Aeropyrum pernix:3353  (5.31) Agrobacterium tumefaciens strain C58 (Cereon):11802  (8.26)
Akodon aerosus:83  (4.61) Aquifex aeolicus:2816  (3.81)
Arabidopsis thaliana:166123  (13.5) Archaeoglobus fulgidus:4326  (3.98)
Artocarpus heterophyllus:2  (0.67) Azotobacter vinelandii:8  (1.33)
Bacillus halodurans:6059  (3.75) Bacillus subtilis:7542  (3.96)
Borrelia burgdorferi:1866  (4.74) Bos taurus:380  (1.12)
Brucella melitensis:4482  (4.87) Buchnera sp. APS:1529  (3.65)
Caenorhabditis elegans:13923  (2.07) Campylobacter jejuni:4283  (4.58)
Candida albicans:5  (1) Canis lupus familiaris:15  (0.79)
Caulobacter crescentus:5910  (4.21) Chlamydia pneumoniae:1589  (3.83)
Chlamydia trachomatis:1537  (3.92) Chlamydomonas reinhardtii:51  (1.46)
Clostridium acetobutylicum:3404  (4.93) Corynebacterium glutamicum:2428  (4.35)
Danio rerio:135  (1.21) Deinococcus radiodurans:3953  (3.33)
Desulfovibrio desulfuricans:4  (0.5) Drosophila melanogaster:42223  (5.03)
Encephalitozoon_cuniculi:3788  (21.65) Enterobacteria phage:44  (0.81)
Enterobacteria phage PRD1:1  (1) Escherichia coli K-12:27152  (7.19)
Escherichia coli O157:8029  (3.32) Escherichia coli O157:H7:7498  (5.25)
Fusobacterium nucleatum:1940  (4.4) Gallus gallus:423  (1.38)
Geobacillus stearothermophilus:14  (0.58) Haemophilus influenzae:3195  (2.93)
Haloarcula marismortui:5  (0.62) Halobacterium sp. NRC-1:4582  (5.94)
Helicobacter pylori 26695:4397  (4.02) Helicobacter pylori J99:2587  (3.8)
Hepatitis C virus:4  (4) Homo sapiens:204997  (12.46)
Human herpesvirus 3:37  (1.68) Human herpesvirus 8:42  (1.62)
Human immunodeficiency virus:52  (0.72) Human immunodeficiency virus 1:29  (1.04)
Human papillomavirus type 16:4  (1) Influenza A virus:14  (1)
Klebsiella pneumoniae:9  (0.82) Lactococcus lactis:2668  (2.96)
Listeria innocua:3819  (4.91) Mesorhizobium loti:15959  (5.96)
Methanocaldococcus jannaschii:3266  (4.65) Methanopyrus kandleri AV19:2696  (7.64)
Methanosarcina acetivorans str.C2A:3444  (6.61) Methanothermobacter thermautotrophicus:3734  (4.13)
Mus musculus:16902  (2.99) Mycobacterium leprae:1859  (3.46)
Mycobacterium tuberculosis:7799  (4.52) Mycobacterium tuberculosis CDC1551:2856  (4.73)
Mycoplasma genitalium:1011  (4) Mycoplasma pneumoniae:1802  (3.57)
Mycoplasma pulmonis:979  (5.56) Neisseria meningitidis MC58:2938  (3.21)
Neisseria meningitidis Z2491:2614  (3.12) Neurospora crassa:3  (1)
Nostoc sp. PCC 7120:4511  (5.31) Oryctolagus cuniculus:36  (0.69)
Oryza sativa:416  (1.05) Pan troglodytes:1  (1)
Paracoccus denitrificans:6  (0.67) Pasteurella multocida:3390  (2.82)
Pisum sativum:3  (0.6) Plasmodium falciparum:1708  (1.81)
Pseudomonas aeruginosa:18846  (6.43) Pseudomonas putida:14  (0.7)
Pyrobaculum aerophilum:3016  (7.54) Pyrococcus abyssi:3436  (4.21)
Pyrococcus furiosus:6  (0.5) Pyrococcus horikoshii:3302  (4.27)
Ralstonia solanacearum:7476  (5.89) Rattus norvegicus:4269  (2.01)
Rhodobacter sphaeroides:1  (1) Rickettsia conorii:1662  (5.05)
Rickettsia prowazekii:1711  (3.86) Rickettsia sibirica 246:116  (1.18)
Saccharomyces cerevisiae:366470  (56.91) Salmonella typhimurium LT2:5659  (4.59)
Schizosaccharomyces pombe:26786  (9.04) Sinorhizobium meliloti:13454  (8.42)
Staphylococcus aureus:11  (0.58) Staphylococcus aureus N315:2697  (4.49)
Streptococcus pneumoniae TIGR4:2455  (4.82) Streptococcus pyogenes:2232  (2.92)
Sulfolobus solfataricus:3052  (6.38) Sus scrofa:48  (0.74)
Synechocystis:7521  (6.24) synthetic construct:23  (0.58)
Thermoplasma acidophilum:2901  (5.35) Thermoplasma volcanium:2959  (5.06)
Thermotoga maritima:3316  (3.3) Thermus aquaticus:3  (0.5)
Thermus thermophilus:68  (0.73) Treponema pallidum:3123  (4.86)
Ureaplasma urealyticum:1030  (3.76) Vibrio cholerae:10771  (5.24)
Xenopus laevis:599  (1.5) Xylella fastidiosa:3733  (3.36)
Yersinia pestis:5603  (5.15)
Aeropyrum pernix
domain fusion(M0036):126 gene neighbourhoods(M0038):271
phylogenetic profile(M0037):2956
Agrobacterium tumefaciens strain C58 (Cereon)
phylogenetic profile(M0037):11802
Akodon aerosus
affinity technology(M0045):13 two hybrid test(M0034):70
Aquifex aeolicus
domain fusion(M0036):282 gene neighbourhoods(M0038):206
phylogenetic profile(M0037):2327 structure based prediction(M0048):2
Arabidopsis thaliana
affinity technology(M0045):2991 anti bait coimmunoprecipitation(M0089):160
anti tag coimmunoprecipitation(M0065):386 Biochemical Activity(M0083):240
blue native page(M0096):75 Chromatin Immunoprecipitation (ChIP)(M0042):1
chromatography technology(M0085):90 Co-fractionation(M0079):83
coimmunoprecipitation(M0010):270 colocalization/visualisation technologies(M0063):4
comigration in non denaturing gel electrophoresis(M0097):1 confocal microscopy(M0094):69
copurification(M0013):43 cosedimentation(M0033):8
cross-linking studies(M0014):6 Dosage Growth Defect(M0082):3
Dosage Rescue(M0077):1 electron microscopy(M0062):10
electrophoretic mobility shift assay(M0098):9 elisa:enzyme-linked immunosorbent assay(M0051):1
enzymatic study(M0066):60 far western blotting(M0060):52
filter binding(M0053):19 fluorescence technology(M0052):393
Gel retardation assays (M0019):2 genetic,synthetic growth effect(M0055):9
Invitro(M0084):294 isothermal titration calorimetry(M0103):5
mass spectrometry studies of complexes(M0028):1 molecular sieving(M0018):60
nuclear magnetic resonance(M0070):2 Other Biophysical(M0031):90
phage display(M0050):99 Phenotypic Enhancement(M0075):45
Phenotypic Suppression(M0080):51 phosphatase assay(M0101):7
Predicted Arabidopsis molecular interactions (PAIR)(M0106):145841 protein array(M0068):664
protein complementation assay(M0069):20 protein kinase assay(M0095):81
Protein-RNA(M0078):3 pull down(M0067):590
Reconstituted Complex(M0074):867 resonance energy transfer(M0061):39
structure based prediction(M0048):28 surface plasmon resonance(M0049):6
Synthetic Rescue(M0081):2 tandem affinity purification(M0088):898
two hybrid test(M0034):11380 ubiquitin reconstruction(M0100):65
x-ray crystallography(M0071):8
Archaeoglobus fulgidus
domain fusion(M0036):798 gene neighbourhoods(M0038):462
phylogenetic profile(M0037):3056 structure based prediction(M0048):9
x-ray crystallography(M0071):2
Artocarpus heterophyllus
structure based prediction(M0048):2
Azotobacter vinelandii
resonance energy transfer(M0061):1 structure based prediction(M0048):7
Bacillus halodurans
gene neighbourhoods(M0038):833 phylogenetic profile(M0037):5225
structure based prediction(M0048):1
Bacillus subtilis
domain fusion(M0036):2213 gene neighbourhoods(M0038):812
phylogenetic profile(M0037):4489 structure based prediction(M0048):29
Borrelia burgdorferi
domain fusion(M0036):41 gene neighbourhoods(M0038):203
phylogenetic profile(M0037):1620 structure based prediction(M0048):2
Bos taurus
affinity technology(M0045):30 anti bait coimmunoprecipitation(M0089):49
Biochemical Activity(M0083):6 Chromatin Immunoprecipitation (ChIP)(M0042):1
chromatography technology(M0085):61 Co-fractionation(M0079):1
coimmunoprecipitation(M0010):10 comigration in non denaturing gel electrophoresis(M0097):1
copurification(M0013):1 cosedimentation(M0033):20
cross-linking studies(M0014):3 electron microscopy(M0062):1
enzymatic study(M0066):3 far western blotting(M0060):2
filter binding(M0053):1 molecular sieving(M0018):6
nuclear magnetic resonance(M0070):2 Other Biophysical(M0031):16
pull down(M0067):23 Reconstituted Complex(M0074):4
structure based prediction(M0048):117 surface plasmon resonance(M0049):2
two hybrid test(M0034):6 x-ray crystallography(M0071):17
Brucella melitensis
phylogenetic profile(M0037):4482
Buchnera sp. APS
gene neighbourhoods(M0038):241 phylogenetic profile(M0037):1288
Caenorhabditis elegans
affinity technology(M0045):451 anti bait coimmunoprecipitation(M0089):56
anti tag coimmunoprecipitation(M0065):149 Biochemical Activity(M0083):14
chromatography technology(M0085):36 Co-fractionation(M0079):1
coimmunoprecipitation(M0010):89 colocalization by immunostaining(M0025):1
colocalization/visualisation technologies(M0063):6 Competition binding(M0012):3
confocal microscopy(M0094):15 copurification(M0013):3
cosedimentation(M0033):2 Dosage Rescue(M0077):5
electron microscopy(M0062):2 enzymatic study(M0066):6
far western blotting(M0060):14 fluorescence technology(M0052):23
Gel retardation assays (M0019):4 imaging techniques(M0072):2
isothermal titration calorimetry(M0103):1 molecular sieving(M0018):16
Negative Genetic(M0104):1242 Other Biophysical(M0031):33
Phenotypic Enhancement(M0075):889 Phenotypic Suppression(M0080):56
protein complementation assay(M0069):3 protein kinase assay(M0095):10
Protein-RNA(M0078):1 pull down(M0067):117
Reconstituted Complex(M0074):38 resonance energy transfer(M0061):1
structure based prediction(M0048):7 surface plasmon resonance(M0049):2
Synthetic Rescue(M0081):112 tandem affinity purification(M0088):8
two hybrid test(M0034):10506 x-ray crystallography(M0071):4
Campylobacter jejuni
domain fusion(M0036):230 gene neighbourhoods(M0038):322
phylogenetic profile(M0037):2558 structure based prediction(M0048):4
two hybrid test(M0034):1170
Candida albicans
molecular sieving(M0018):1 structure based prediction(M0048):1
two hybrid test(M0034):3
Canis lupus familiaris
affinity technology(M0045):5 coimmunoprecipitation(M0010):1
colocalization/visualisation technologies(M0063):1 cosedimentation(M0033):1
cross-linking studies(M0014):1 molecular sieving(M0018):1
Other Biophysical(M0031):1 pull down(M0067):2
Reconstituted Complex(M0074):1 resonance energy transfer(M0061):1
structure based prediction(M0048):2 two hybrid test(M0034):1
Caulobacter crescentus
anti tag coimmunoprecipitation(M0065):1 cross-linking studies(M0014):2
gene neighbourhoods(M0038):565 molecular sieving(M0018):1
phylogenetic profile(M0037):5339 protein kinase assay(M0095):2
Chlamydia pneumoniae
domain fusion(M0036):103 gene neighbourhoods(M0038):171
phylogenetic profile(M0037):1315
Chlamydia trachomatis
domain fusion(M0036):57 gene neighbourhoods(M0038):167
phylogenetic profile(M0037):1313
Chlamydomonas reinhardtii
affinity technology(M0045):11 anti bait coimmunoprecipitation(M0089):18
anti tag coimmunoprecipitation(M0065):1 Biochemical Activity(M0083):6
far western blotting(M0060):1 nuclear magnetic resonance(M0070):1
pull down(M0067):7 structure based prediction(M0048):6
Clostridium acetobutylicum
phylogenetic profile(M0037):3403 structure based prediction(M0048):1
Corynebacterium glutamicum
phylogenetic profile(M0037):2427 structure based prediction(M0048):1
Danio rerio
affinity technology(M0045):41 Biochemical Activity(M0083):5
coimmunoprecipitation(M0010):3 Gel retardation assays (M0019):1
Other Biophysical(M0031):1 Phenotypic Enhancement(M0075):43
Phenotypic Suppression(M0080):23 pull down(M0067):1
Reconstituted Complex(M0074):13 solid phase assay(M0102):1
structure based prediction(M0048):1 two hybrid test(M0034):3
Deinococcus radiodurans
domain fusion(M0036):1204 gene neighbourhoods(M0038):415
phylogenetic profile(M0037):2331 structure based prediction(M0048):3
Desulfovibrio desulfuricans
structure based prediction(M0048):4
Drosophila melanogaster
affinity technology(M0045):11357 anti bait coimmunoprecipitation(M0089):158
anti tag coimmunoprecipitation(M0065):306 Biochemical Activity(M0083):55
chromatography technology(M0085):15 Co-fractionation(M0079):51
coimmunoprecipitation(M0010):136 colocalization by immunostaining(M0025):2
colocalization/visualisation technologies(M0063):18 comigration in non denaturing gel electrophoresis(M0097):4
Competition binding(M0012):11 confocal microscopy(M0094):7
copurification(M0013):57 cosedimentation(M0033):24
cross-linking studies(M0014):12 Dosage Rescue(M0077):3
electron microscopy(M0062):7 electrophoretic mobility shift assay(M0098):2
elisa:enzyme-linked immunosorbent assay(M0051):4 enzymatic study(M0066):6
far western blotting(M0060):17 filter binding(M0053):2
fluorescence technology(M0052):25 Gel retardation assays (M0019):8
genetic,synthetic growth effect(M0055):3 imaging techniques(M0072):12
inferred by curator(M0093):9 isothermal titration calorimetry(M0103):1
molecular sieving(M0018):26 nuclear magnetic resonance(M0070):4
Other Biophysical(M0031):918 phage display(M0050):14
Phenotypic Enhancement(M0075):1411 Phenotypic Suppression(M0080):1815
phosphatase assay(M0101):2 protein complementation assay(M0069):3
protein kinase assay(M0095):8 Protein-RNA(M0078):1
pull down(M0067):205 Reconstituted Complex(M0074):200
solid phase assay(M0102):1 structure based prediction(M0048):30
surface plasmon resonance(M0049):8 Synthetic Haploinsufficiency(M0086):2
Synthetic Rescue(M0081):11 tandem affinity purification(M0088):20
two hybrid test(M0034):25230 western blot(M0021):1
x-ray crystallography(M0071):9
Encephalitozoon_cuniculi
phylogenetic profile(M0037):3788
Enterobacteria phage
cross-linking studies(M0014):1 electron microscopy(M0062):1
molecular sieving(M0018):1 structure based prediction(M0048):4
two hybrid test(M0034):38 x-ray crystallography(M0071):1
Enterobacteria phage PRD1
structure based prediction(M0048):1
Escherichia coli K-12
affinity technology(M0045):12 anti bait coimmunoprecipitation(M0089):2
anti tag coimmunoprecipitation(M0065):1774 Biochemical Activity(M0083):92
blue native page(M0096):3 chromatography technology(M0085):118
coimmunoprecipitation(M0010):111 colocalization by immunostaining(M0025):1
comigration in non denaturing gel electrophoresis(M0097):61 cosedimentation(M0033):84
cross-linking studies(M0014):222 domain fusion(M0036):2697
electron microscopy(M0062):35 elisa:enzyme-linked immunosorbent assay(M0051):2
enzymatic study(M0066):17 far western blotting(M0060):7
filter binding(M0053):4 fluorescence technology(M0052):11
Gel retardation assays (M0019):28 gene neighbourhoods(M0038):1019
inferred by author(M0091):5897 isothermal titration calorimetry(M0103):4
mass spectrometry studies of complexes(M0028):31 molecular sieving(M0018):155
nuclear magnetic resonance(M0070):9 Other Biophysical(M0031):83
Phenotypic Enhancement(M0075):4 phosphatase assay(M0101):1
phylogenetic profile(M0037):5127 protein complementation assay(M0069):114
protein kinase assay(M0095):2 pull down(M0067):37
Reconstituted Complex(M0074):1 resonance energy transfer(M0061):7
structure based prediction(M0048):795 surface plasmon resonance(M0049):45
tandem affinity purification(M0088):8282 two hybrid test(M0034):143
x-ray crystallography(M0071):121
Escherichia coli O157
gene neighbourhoods(M0038):1295 phylogenetic profile(M0037):6734
Escherichia coli O157:H7
anti tag coimmunoprecipitation(M0065):596 blue native page(M0096):1
cosedimentation(M0033):1 electron microscopy(M0062):1
elisa:enzyme-linked immunosorbent assay(M0051):1 molecular sieving(M0018):4
Other Biophysical(M0031):3 phylogenetic profile(M0037):6881
pull down(M0067):7 two hybrid test(M0034):1
x-ray crystallography(M0071):4
Fusobacterium nucleatum
phylogenetic profile(M0037):1940
Gallus gallus
affinity technology(M0045):154 anti bait coimmunoprecipitation(M0089):86
anti tag coimmunoprecipitation(M0065):17 Biochemical Activity(M0083):4
chromatography technology(M0085):2 Co-fractionation(M0079):3
coimmunoprecipitation(M0010):15 colocalization by immunostaining(M0025):2
colocalization/visualisation technologies(M0063):2 cosedimentation(M0033):2
cross-linking studies(M0014):6 elisa:enzyme-linked immunosorbent assay(M0051):1
far western blotting(M0060):2 fluorescence technology(M0052):4
Gel retardation assays (M0019):4 genetic,synthetic growth effect(M0055):2
genetic,synthetic lethality(M0047):1 molecular sieving(M0018):7
Other Biophysical(M0031):6 phage display(M0050):2
Phenotypic Enhancement(M0075):4 protein kinase assay(M0095):1
pull down(M0067):10 Reconstituted Complex(M0074):15
solid phase assay(M0102):2 structure based prediction(M0048):52
surface plasmon resonance(M0049):1 transient coexpression(M0039):1
two hybrid test(M0034):6 x-ray crystallography(M0071):11
Geobacillus stearothermophilus
Other Biophysical(M0031):2 structure based prediction(M0048):13
Haemophilus influenzae
domain fusion(M0036):194 gene neighbourhoods(M0038):527
phylogenetic profile(M0037):2424 structure based prediction(M0048):48
x-ray crystallography(M0071):2
Haloarcula marismortui
structure based prediction(M0048):5
Halobacterium sp. NRC-1
gene neighbourhoods(M0038):327 phylogenetic profile(M0037):4255
Helicobacter pylori 26695
cosedimentation(M0033):1 domain fusion(M0036):202
gene neighbourhoods(M0038):233 molecular sieving(M0018):1
Other Biophysical(M0031):1434 phylogenetic profile(M0037):2267
pull down(M0067):3 structure based prediction(M0048):1
two hybrid test(M0034):256 x-ray crystallography(M0071):1
Helicobacter pylori J99
blue native page(M0096):28 domain fusion(M0036):132
gene neighbourhoods(M0038):235 phylogenetic profile(M0037):2193
Hepatitis C virus
affinity technology(M0045):1 coimmunoprecipitation(M0010):1
colocalization by immunostaining(M0025):1 colocalization/visualisation technologies(M0063):1
cross-linking studies(M0014):1 far western blotting(M0060):1
structure based prediction(M0048):1 two hybrid test(M0034):1
Homo sapiens
affinity technology(M0045):53392 anti bait coimmunoprecipitation(M0089):9383
anti tag coimmunoprecipitation(M0065):12522 Biochemical Activity(M0083):3496
blue native page(M0096):76 Chromatin Immunoprecipitation (ChIP)(M0042):399
chromatography technology(M0085):853 Co-fractionation(M0079):639
coimmunoprecipitation(M0010):4407 colocalization by immunostaining(M0025):225
colocalization/visualisation technologies(M0063):955 comigration in non denaturing gel electrophoresis(M0097):64
Competition binding(M0012):95 confocal microscopy(M0094):472
copurification(M0013):1149 cosedimentation(M0033):591
cross-linking studies(M0014):3734 dihydrofolate reductase reconstruction(M0090):6
Dosage Lethality(M0076):101 Dosage Rescue(M0077):5
electron microscopy(M0062):41 electrophoretic mobility shift assay(M0098):20
elisa:enzyme-linked immunosorbent assay(M0051):154 enzymatic study(M0066):400
far western blotting(M0060):615 filter binding(M0053):197
fluorescence technology(M0052):717 Gel retardation assays (M0019):28
genetic,synthetic growth effect(M0055):4 genetic,synthetic lethality(M0047):1
imaging techniques(M0072):67 inferred by curator(M0093):193
Invitro(M0084):19273 Invivo(M0073):17846
isothermal titration calorimetry(M0103):105 mass spectrometry studies of complexes(M0028):30
molecular sieving(M0018):498 Negative Genetic(M0104):605
nuclear magnetic resonance(M0070):119 Other Biophysical(M0031):1528
other genetic(M0030):27 peptide array(M0092):986
phage display(M0050):137 Phenotypic Enhancement(M0075):162
Phenotypic Suppression(M0080):136 phosphatase assay(M0101):194
Positive Genetic(M0105):271 protein array(M0068):556
protein complementation assay(M0069):67 protein kinase assay(M0095):411
Protein-RNA(M0078):35 pull down(M0067):5301
Reconstituted Complex(M0074):12876 resonance energy transfer(M0061):90
solid phase assay(M0102):26 structure based prediction(M0048):984
surface plasmon resonance(M0049):356 Synthetic Rescue(M0081):8
tandem affinity purification(M0088):3558 TFSVM (Predicted TF by SVM)(M0064):11248
transient coexpression(M0039):1 two hybrid test(M0034):32005
ubiquitin reconstruction(M0100):97 western blot(M0021):1
x-ray crystallography(M0071):473
Human herpesvirus 3
structure based prediction(M0048):1 two hybrid test(M0034):37
Human herpesvirus 8
anti tag coimmunoprecipitation(M0065):17 Other Biophysical(M0031):1
two hybrid test(M0034):24
Human immunodeficiency virus
affinity technology(M0045):2 coimmunoprecipitation(M0010):1
cosedimentation(M0033):1 cross-linking studies(M0014):2
Other Biophysical(M0031):2 resonance energy transfer(M0061):1
structure based prediction(M0048):45 two hybrid test(M0034):1
Human immunodeficiency virus 1
affinity technology(M0045):6 Biochemical Activity(M0083):1
coimmunoprecipitation(M0010):2 cross-linking studies(M0014):2
fluorescence technology(M0052):2 Other Biophysical(M0031):1
Reconstituted Complex(M0074):2 structure based prediction(M0048):14
two hybrid test(M0034):1
Human papillomavirus type 16
coimmunoprecipitation(M0010):1 cosedimentation(M0033):1
nuclear magnetic resonance(M0070):1 pull down(M0067):1
two hybrid test(M0034):2
Influenza A virus
structure based prediction(M0048):14
Klebsiella pneumoniae
structure based prediction(M0048):9
Lactococcus lactis
gene neighbourhoods(M0038):412 Other Biophysical(M0031):2
phylogenetic profile(M0037):2253 structure based prediction(M0048):2
Listeria innocua
phylogenetic profile(M0037):3818 structure based prediction(M0048):1
Mesorhizobium loti
gene neighbourhoods(M0038):1185 phylogenetic profile(M0037):14773
structure based prediction(M0048):1
Methanocaldococcus jannaschii
cosedimentation(M0033):3 domain fusion(M0036):299
electron microscopy(M0062):3 gene neighbourhoods(M0038):205
molecular sieving(M0018):1 phylogenetic profile(M0037):2738
pull down(M0067):3 structure based prediction(M0048):11
two hybrid test(M0034):1 x-ray crystallography(M0071):2
Methanopyrus kandleri AV19
phylogenetic profile(M0037):2696
Methanosarcina acetivorans str.C2A
phylogenetic profile(M0037):3444
Methanothermobacter thermautotrophicus
blue native page(M0096):43 domain fusion(M0036):591
Gel retardation assays (M0019):1 gene neighbourhoods(M0038):347
mass spectrometry studies of complexes(M0028):1 molecular sieving(M0018):2
Other Biophysical(M0031):2 phylogenetic profile(M0037):2728
structure based prediction(M0048):16 surface plasmon resonance(M0049):1
two hybrid test(M0034):2 x-ray crystallography(M0071):2
Mus musculus
affinity technology(M0045):4159 anti bait coimmunoprecipitation(M0089):1428
anti tag coimmunoprecipitation(M0065):1581 Biochemical Activity(M0083):214
blue native page(M0096):63 Chromatin Immunoprecipitation (ChIP)(M0042):241
chromatography technology(M0085):354 Co-fractionation(M0079):63
coimmunoprecipitation(M0010):1260 colocalization by immunostaining(M0025):36
colocalization/visualisation technologies(M0063):113 comigration in non denaturing gel electrophoresis(M0097):5
Competition binding(M0012):43 confocal microscopy(M0094):98
copurification(M0013):50 cosedimentation(M0033):759
cross-linking studies(M0014):389 Dosage Rescue(M0077):4
electron microscopy(M0062):7 electrophoretic mobility shift assay(M0098):6
elisa:enzyme-linked immunosorbent assay(M0051):21 enzymatic study(M0066):38
far western blotting(M0060):74 filter binding(M0053):14
fluorescence technology(M0052):99 Gel retardation assays (M0019):17
genetic,synthetic growth effect(M0055):7 genetic,synthetic lethality(M0047):12
imaging techniques(M0072):11 inferred by curator(M0093):83
isothermal titration calorimetry(M0103):15 mass spectrometry studies of complexes(M0028):3
molecular sieving(M0018):83 nuclear magnetic resonance(M0070):7
Other Biophysical(M0031):577 other genetic(M0030):8
peptide array(M0092):2 phage display(M0050):16
Phenotypic Enhancement(M0075):76 Phenotypic Suppression(M0080):83
protein array(M0068):1 protein complementation assay(M0069):1
protein kinase assay(M0095):7 Protein-RNA(M0078):2
pull down(M0067):980 Reconstituted Complex(M0074):469
resonance energy transfer(M0061):7 solid phase assay(M0102):2
structure based prediction(M0048):355 surface plasmon resonance(M0049):49
Synthetic Haploinsufficiency(M0086):2 Synthetic Rescue(M0081):7
tandem affinity purification(M0088):153 transient coexpression(M0039):1
two hybrid test(M0034):2699 ubiquitin reconstruction(M0100):1
x-ray crystallography(M0071):59
Mycobacterium leprae
gene neighbourhoods(M0038):220 phylogenetic profile(M0037):1638
structure based prediction(M0048):1
Mycobacterium tuberculosis
affinity technology(M0045):4 domain fusion(M0036):4292
gene neighbourhoods(M0038):574 Other Biophysical(M0031):4
phylogenetic profile(M0037):2863 structure based prediction(M0048):56
two hybrid test(M0034):7
Mycobacterium tuberculosis CDC1551
phylogenetic profile(M0037):2855 structure based prediction(M0048):1
Mycoplasma genitalium
domain fusion(M0036):24 gene neighbourhoods(M0038):131
phylogenetic profile(M0037):856
Mycoplasma pneumoniae
gene neighbourhoods(M0038):147 inferred by author(M0091):343
phylogenetic profile(M0037):791 structure based prediction(M0048):1
tandem affinity purification(M0088):521
Mycoplasma pulmonis
phylogenetic profile(M0037):979
Neisseria meningitidis MC58
domain fusion(M0036):222 gene neighbourhoods(M0038):339
phylogenetic profile(M0037):2377 structure based prediction(M0048):1
Neisseria meningitidis Z2491
gene neighbourhoods(M0038):345 phylogenetic profile(M0037):2269
Neurospora crassa
enzymatic study(M0066):1 two hybrid test(M0034):1
x-ray crystallography(M0071):1
Nostoc sp. PCC 7120
molecular sieving(M0018):1 phylogenetic profile(M0037):4508
structure based prediction(M0048):1 x-ray crystallography(M0071):1
Oryctolagus cuniculus
Biochemical Activity(M0083):1 coimmunoprecipitation(M0010):3
comigration in non denaturing gel electrophoresis(M0097):1 copurification(M0013):1
cross-linking studies(M0014):1 electron microscopy(M0062):1
enzymatic study(M0066):1 filter binding(M0053):2
Gel retardation assays (M0019):1 molecular sieving(M0018):1
nuclear magnetic resonance(M0070):1 Other Biophysical(M0031):1
phage display(M0050):1 protein kinase assay(M0095):1
pull down(M0067):1 Reconstituted Complex(M0074):4
resonance energy transfer(M0061):1 structure based prediction(M0048):13
two hybrid test(M0034):1 x-ray crystallography(M0071):2
Oryza sativa
affinity technology(M0045):3 anti bait coimmunoprecipitation(M0089):4
anti tag coimmunoprecipitation(M0065):2 coimmunoprecipitation(M0010):2
fluorescence technology(M0052):1 molecular sieving(M0018):3
pull down(M0067):3 structure based prediction(M0048):1
two hybrid test(M0034):396 x-ray crystallography(M0071):1
Pan troglodytes
structure based prediction(M0048):1
Paracoccus denitrificans
structure based prediction(M0048):6
Pasteurella multocida
gene neighbourhoods(M0038):690 phylogenetic profile(M0037):2700
Pisum sativum
cross-linking studies(M0014):1 structure based prediction(M0048):2
Plasmodium falciparum
Biochemical Activity(M0083):1 enzymatic study(M0066):4
molecular sieving(M0018):2 structure based prediction(M0048):4
two hybrid test(M0034):1696 x-ray crystallography(M0071):2
Pseudomonas aeruginosa
cross-linking studies(M0014):1 domain fusion(M0036):6577
gene neighbourhoods(M0038):1382 Other Biophysical(M0031):3
phylogenetic profile(M0037):10828 structure based prediction(M0048):53
two hybrid test(M0034):1 x-ray crystallography(M0071):2
Pseudomonas putida
structure based prediction(M0048):13 x-ray crystallography(M0071):1
Pyrobaculum aerophilum
phylogenetic profile(M0037):3015 structure based prediction(M0048):1
Pyrococcus abyssi
domain fusion(M0036):224 gene neighbourhoods(M0038):387
phylogenetic profile(M0037):2824 structure based prediction(M0048):1
Pyrococcus furiosus
coimmunoprecipitation(M0010):1 structure based prediction(M0048):5
Pyrococcus horikoshii
anti bait coimmunoprecipitation(M0089):2 domain fusion(M0036):231
gene neighbourhoods(M0038):307 Other Biophysical(M0031):1
phylogenetic profile(M0037):2749 structure based prediction(M0048):8
two hybrid test(M0034):5
Ralstonia solanacearum
phylogenetic profile(M0037):7476
Rattus norvegicus
affinity technology(M0045):1056 anti bait coimmunoprecipitation(M0089):206
anti tag coimmunoprecipitation(M0065):654 Biochemical Activity(M0083):39
blue native page(M0096):6 chromatography technology(M0085):2
Co-fractionation(M0079):34 coimmunoprecipitation(M0010):618
colocalization by immunostaining(M0025):19 colocalization/visualisation technologies(M0063):54
comigration in non denaturing gel electrophoresis(M0097):4 Competition binding(M0012):20
confocal microscopy(M0094):36 copurification(M0013):24
cosedimentation(M0033):49 cross-linking studies(M0014):22
electron microscopy(M0062):5 elisa:enzyme-linked immunosorbent assay(M0051):2
enzymatic study(M0066):6 far western blotting(M0060):55
filter binding(M0053):18 fluorescence technology(M0052):33
Gel retardation assays (M0019):3 imaging techniques(M0072):5
inferred by curator(M0093):25 isothermal titration calorimetry(M0103):3
molecular sieving(M0018):14 nuclear magnetic resonance(M0070):7
Other Biophysical(M0031):143 other genetic(M0030):2
peptide array(M0092):1 phage display(M0050):4
Phenotypic Enhancement(M0075):1 Phenotypic Suppression(M0080):3
protein array(M0068):2 protein kinase assay(M0095):8
pull down(M0067):342 Reconstituted Complex(M0074):155
resonance energy transfer(M0061):11 solid phase assay(M0102):1
structure based prediction(M0048):102 surface plasmon resonance(M0049):11
two hybrid test(M0034):442 x-ray crystallography(M0071):32
Rhodobacter sphaeroides
structure based prediction(M0048):1
Rickettsia conorii
Other Biophysical(M0031):1 phylogenetic profile(M0037):1661
Rickettsia prowazekii
domain fusion(M0036):48 gene neighbourhoods(M0038):165
phylogenetic profile(M0037):1498
Rickettsia sibirica 246
two hybrid test(M0034):116
Saccharomyces cerevisiae
Affinity Column(M0006):33 Affinity Precipitation(M0044):63
affinity technology(M0045):55762 anti bait coimmunoprecipitation(M0089):307
anti tag coimmunoprecipitation(M0065):14344 Bayesian Predicted Interaction(M0046):15918
Biochemical Activity(M0083):5716 blue native page(M0096):36
Chromatin Immunoprecipitation (ChIP)(M0042):4370 chromatography technology(M0085):1602
Co-fractionation(M0079):773 coimmunoprecipitation(M0010):1742
colocalization by immunostaining(M0025):6 colocalization/visualisation technologies(M0063):499
comigration in non denaturing gel electrophoresis(M0097):87 Competition binding(M0012):9
confocal microscopy(M0094):14 copurification(M0013):1491
cosedimentation(M0033):237 cross-linking studies(M0014):177
dihydrofolate reductase reconstruction(M0090):9384 domain fusion(M0036):3017
Dosage Growth Defect(M0082):1901 Dosage Lethality(M0076):961
Dosage Rescue(M0077):4192 electron microscopy(M0062):76
electrophoretic mobility shift assay(M0098):5 elisa:enzyme-linked immunosorbent assay(M0051):15
enzymatic study(M0066):38 far western blotting(M0060):96
filter binding(M0053):42 fluorescence technology(M0052):194
Gel retardation assays (M0019):34 gene neighbourhoods(M0038):9
genetic,conditional synthetic lethal(M0054):15 genetic suppression(M0058):210
genetic,suppression expression alteration(M0057):145 genetic,suppression mutation(M0056):39
genetic,synthetic growth effect(M0055):19376 genetic,synthetic lethality(M0047):11536
genetic synthetic phenotype(M0059):46 genetic,tetrad analysis(M0020):1318
inferred by author(M0091):4230 inferred by curator(M0093):716
In vitro binding(M0026):26 isothermal titration calorimetry(M0103):9
mass spectrometry studies of complexes(M0028):21 molecular sieving(M0018):296
Negative Genetic(M0104):84024 nuclear magnetic resonance(M0070):12
Other Biophysical(M0031):7285 other genetic(M0030):143
peptide array(M0092):182 phage display(M0050):18
Phenotypic Enhancement(M0075):4883 Phenotypic Suppression(M0080):4024
phosphatase assay(M0101):6 phylogenetic profile(M0037):8302
Positive Genetic(M0105):18914 protein array(M0068):526
protein complementation assay(M0069):31 protein kinase assay(M0095):216
Protein-RNA(M0078):514 pull down(M0067):1863
Reconstituted Complex(M0074):4152 resonance energy transfer(M0061):17
solid phase assay(M0102):5 structure based prediction(M0048):579
surface plasmon resonance(M0049):62 Synthetic Haploinsufficiency(M0086):263
Synthetic Rescue(M0081):4308 tandem affinity purification(M0088):43996
Transcription Factor(M0041):238 transient coexpression(M0039):98
two hybrid test(M0034):18500 ubiquitin reconstruction(M0100):2076
x-ray crystallography(M0071):115
Salmonella typhimurium LT2
affinity technology(M0045):1 chromatography technology(M0085):2
coimmunoprecipitation(M0010):2 cosedimentation(M0033):2
cross-linking studies(M0014):1 molecular sieving(M0018):1
peptide array(M0092):1 phylogenetic profile(M0037):5625
pull down(M0067):3 structure based prediction(M0048):15
two hybrid test(M0034):3 x-ray crystallography(M0071):4
Schizosaccharomyces pombe
affinity technology(M0045):3096 anti bait coimmunoprecipitation(M0089):17
anti tag coimmunoprecipitation(M0065):63 Biochemical Activity(M0083):222
chromatography technology(M0085):1 Co-fractionation(M0079):42
coimmunoprecipitation(M0010):49 colocalization/visualisation technologies(M0063):262
copurification(M0013):100 cosedimentation(M0033):2
cross-linking studies(M0014):5 Dosage Growth Defect(M0082):31
Dosage Lethality(M0076):68 Dosage Rescue(M0077):568
enzymatic study(M0066):4 far western blotting(M0060):27
fluorescence technology(M0052):7 genetic,synthetic growth effect(M0055):1534
genetic,synthetic lethality(M0047):659 isothermal titration calorimetry(M0103):3
molecular sieving(M0018):7 Negative Genetic(M0104):6758
Other Biophysical(M0031):30 Phenotypic Enhancement(M0075):805
Phenotypic Suppression(M0080):649 phylogenetic profile(M0037):8122
Positive Genetic(M0105):1969 protein kinase assay(M0095):1
Protein-RNA(M0078):5 pull down(M0067):42
Reconstituted Complex(M0074):325 structure based prediction(M0048):30
surface plasmon resonance(M0049):1 Synthetic Rescue(M0081):528
tandem affinity purification(M0088):106 two hybrid test(M0034):650
x-ray crystallography(M0071):3
Sinorhizobium meliloti
phylogenetic profile(M0037):13453 structure based prediction(M0048):1
Staphylococcus aureus
Other Biophysical(M0031):1 structure based prediction(M0048):10
Staphylococcus aureus N315
phylogenetic profile(M0037):2697
Streptococcus pneumoniae TIGR4
affinity technology(M0045):132 coimmunoprecipitation(M0010):7
phylogenetic profile(M0037):2316 structure based prediction(M0048):1
Streptococcus pyogenes
gene neighbourhoods(M0038):354 phylogenetic profile(M0037):1871
structure based prediction(M0048):7
Sulfolobus solfataricus
phylogenetic profile(M0037):3043 structure based prediction(M0048):7
x-ray crystallography(M0071):2
Sus scrofa
affinity technology(M0045):15 anti tag coimmunoprecipitation(M0065):1
coimmunoprecipitation(M0010):2 cross-linking studies(M0014):1
molecular sieving(M0018):1 Reconstituted Complex(M0074):1
structure based prediction(M0048):26 x-ray crystallography(M0071):1
Synechocystis
anti bait coimmunoprecipitation(M0089):1 blue native page(M0096):1
cosedimentation(M0033):1 domain fusion(M0036):4563
gene neighbourhoods(M0038):272 isothermal titration calorimetry(M0103):1
molecular sieving(M0018):3 phylogenetic profile(M0037):2663
pull down(M0067):2 structure based prediction(M0048):2
two hybrid test(M0034):12 x-ray crystallography(M0071):1
synthetic construct
structure based prediction(M0048):23
Thermoplasma acidophilum
gene neighbourhoods(M0038):216 phylogenetic profile(M0037):2680
structure based prediction(M0048):5
Thermoplasma volcanium
gene neighbourhoods(M0038):290 phylogenetic profile(M0037):2669
Thermotoga maritima
comigration in non denaturing gel electrophoresis(M0097):1 cosedimentation(M0033):1
cross-linking studies(M0014):2 domain fusion(M0036):529
gene neighbourhoods(M0038):473 phylogenetic profile(M0037):2256
structure based prediction(M0048):51 surface plasmon resonance(M0049):1
x-ray crystallography(M0071):2
Thermus aquaticus
structure based prediction(M0048):3
Thermus thermophilus
structure based prediction(M0048):68
Treponema pallidum
anti tag coimmunoprecipitation(M0065):3 domain fusion(M0036):54
gene neighbourhoods(M0038):203 phylogenetic profile(M0037):1698
structure based prediction(M0048):1 two hybrid test(M0034):1164
Ureaplasma urealyticum
domain fusion(M0036):32 gene neighbourhoods(M0038):141
phylogenetic profile(M0037):857
Vibrio cholerae
domain fusion(M0036):4551 gene neighbourhoods(M0038):1075
phylogenetic profile(M0037):5131 structure based prediction(M0048):14
x-ray crystallography(M0071):1
Xenopus laevis
affinity technology(M0045):235 anti bait coimmunoprecipitation(M0089):41
anti tag coimmunoprecipitation(M0065):23 Biochemical Activity(M0083):31
chromatography technology(M0085):6 Co-fractionation(M0079):17
coimmunoprecipitation(M0010):40 colocalization/visualisation technologies(M0063):7
comigration in non denaturing gel electrophoresis(M0097):1 Competition binding(M0012):2
cosedimentation(M0033):8 cross-linking studies(M0014):14
electron microscopy(M0062):2 enzymatic study(M0066):2
far western blotting(M0060):2 fluorescence technology(M0052):1
Gel retardation assays (M0019):1 imaging techniques(M0072):1
inferred by curator(M0093):6 molecular sieving(M0018):4
nuclear magnetic resonance(M0070):1 Other Biophysical(M0031):7
Phenotypic Suppression(M0080):6 protein complementation assay(M0069):2
protein kinase assay(M0095):3 pull down(M0067):34
Reconstituted Complex(M0074):62 structure based prediction(M0048):17
two hybrid test(M0034):23 x-ray crystallography(M0071):4
Xylella fastidiosa
domain fusion(M0036):357 gene neighbourhoods(M0038):528
phylogenetic profile(M0037):2848
Yersinia pestis
phylogenetic profile(M0037):5602 structure based prediction(M0048):1

 

*This total number excludes the number of interactions extracts from the KEGG pathways; otherwise, the number shall >1M.